The border between perirhinal cortex area 35 nd 36 is characterized by an emerging differentiation between layers II and III in PER36 which is absent in PER35. Cells in layer II of PER36 are slightly more intensely stained and form a regularly packed dense cell layer. Cells in layer III of PER36 show a slightly less regular pattern compared to PER35. Layer V of PER35 is characterized by fairly large heart shaped pyramidal cells that in PER36 are much less abundant. Finally, whereas PER35 is agranular, i.e. lacks a clear granular layer IV, PER36 does express a layer IV although this is only well developed at more dorsal levels of PER36 such that at the border with PER35 this feature is not particularly obvious. Additional immunostains for parvalbumin and calbindin do not provide additional information to recognize this border.
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