The medial border of EC with PaS features a striking merging of layers II and III in PaS, so that the layers II and III are difficult to distinguish here. The darkly stained cells in layer II of the EC tend to be clustered (as seen in sections stained for NeuN), and the deepest cells are strongly positive for calbindin. In the ventral part of PaS, an overall homogeneous but weak staining for calbindin is present superficially, while sparsely distributed cells stained for calbindin are seen in layers III and V. Although not shown in this atlas, the EC / PaS boundary coincides with a marked increase in staining for acetylcholinesterase in layers I-III in parasubiculum. Staining for parvalbumin is not a distinguishing feature for this border.
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