The anterior border of EC is indicated by a reduction in the number of cell layers from six (molecular layer, cell layers II, III, V, and VI, and the lamina dissecans) to three (molecular layer, principal cell layer (layer II) and polymorph layer (layer III)). While this border is not easily identified in sections stained for parvalbumin, the anterior portions of EC stain less densely for calbindin compared to the neighboring olfactory and periamygdaloid areas.
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