The border between CA2 and CA1 coincides with a sudden drop in the size of neurons in the principal cell layer in CA1, which can be observed in sections stained for NeuN. In addition, in CA1, pyramidal cells in the most superficial layer stain positive for calbindin and there is a salient calbindin positive band in the neuropil, at the border between stratum radiatum and stratum lacunosum-moleculare, this band is completely absent in CA2. Finally, in CA1, the neuropil of stratum lacunosum-moleculare stains positive for parvalbumin, which is not seen in CA2.
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