These two parts of the Ammon’s horn are characterized by presence of large pyramidal cells, that form a few closely packed layers on top of one another. Both areas have a layered appearance, similar to CA1 and the subiculum. In the rat the following features differentiate CA3 clearly from CA2: The pyramidal cell layer of CA2 tends to be slightly thicker than in the adjacent CA3, and is populated by a mixture of large cells, similar in size as the CA3 neurons, and small neurons, similar in size to CA1 neurons. CA3 is characterized by the strong calbindin positive mossy fiber input, which is almost completely absent in CA2. Using a stain for parvalbumin, it is obvious that CA2 contains a much higher density of fairly large positive neurons than CA3.
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